AI334 and AQ806 antibodies recognize the spike S protein from SARS-CoV-2 by ELISA

Philippe Hammel; Anna Marchetti; Wanessa C Lima; Kelvin Lau; Florence Pojer; David Hacker; Pierre Cosson

doi:10.24450/journals/abrep.2020.e186

Authors

Philippe Hammel
Anna Marchetti
Wanessa C Lima
Kelvin Lau
Florence Pojer
David Hacker
Pierre Cosson

DOI:

https://doi.org/10.24450/journals/abrep.2020.e186

Abstract

We tested 10 recombinant antibodies directed against the spike S protein from SARS-CoV-1. Among them, antibodies AI334 and AQ806 detect by ELISA the spike S protein from SARS-CoV-2.

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References

Berry JD, Jones S, Drebot MA, et al. Development and characterization of neutralizing monoclonal antibody to the SARS-coronavirus. J Virol Methods. 2004; 120:87-96. PMID:15234813

Coughlin M, Lou G, Martinez O, et al. Generation and characterization of human monoclonal neutralizing antibodies with distinct binding and sequence features against SARS coronavirus using XenoMouse. Virology. 2007; 361:93-102. PMID:17161858

Prabakaran P, Gan J, Feng Y, et al. Structure of severe acute respiratory syndrome coronavirus receptor-binding domain complexed with neutralizing antibody. J Biol Chem. 2006; 281:15829-36. PMID:16597622.

Rockx B, Corti D, Donaldson E, et al. Structural basis for potent cross-neutralizing human monoclonal antibody protection against lethal human and zoonotic severe acute respiratory syndrome coronavirus challenge. J Virol. 2008; 82:3220-35. PMID:18199635

Sui J, Li W, Murakami A, et al. Potent neutralization of severe acute respiratory syndrome (SARS) coronavirus by a human mAb to S1 protein that blocks receptor association. Proc Natl Acad Sci USA. 2004; 101:2536-41. PMID:14983044

ter Meulen J, van den Brink EN, Poon LL, et al. Human monoclonal antibody combination against SARS coronavirus: synergy and coverage of escape mutants. PLoS Med. 2006; 3:e237. PMID:16796401

van den Brink EN, Ter Meulen J, Cox F, et al. Molecular and biological characterization of human monoclonal antibodies binding to the spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus. J Virol. 2005; 79:1635-44. PMID:15650189

Wrapp D, De Vlieger D, Corbett KS, et al. Structural basis for potent neutralization of betacoronaviruses by single-domain camelid antibodies. Cell 2020a; pii:S0092-8674(20)30494-3. PMID:32375025

Wrapp D, Wang N, Corbett KS, et al. Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation. Science. 2020b; 367:1260-1263. PMID:32075877

Yan R, Zhang Y, Li Y, Xia L, Guo Y, Zhou Q. Structural basis for the recognition of SARS-CoV-2 by full-length human ACE2. Science. 2020; 367:1444-1448. PMID:32132184

Yuan M, Wu NC, Zhu X, et al. A highly conserved cryptic epitope in the receptor-binding domains of SARS-CoV-2 and SARS-CoV. Science. 2020. pii:eabb7269. PMID: 32245784