https://oap.unige.ch/journals/abrep/issue/feedAntibody Reports2022-04-12T06:41:19+00:00Prof. Pierre CossonAntibodyReports@unige.chOpen Journal Systems<p><strong>Antibody Reports</strong> (eISSN 2624-8557) is an international, peer-reviewed open access online journal edited by the <a href="https://www.unige.ch/medecine/antibodies/" target="_blank" rel="noopener">Geneva Antibody Facility</a>, designed to focus on technical, experimental validation of recombinant antibodies. Our aim is to encourage scientists to publish their experimental results in as much detail as possible, with the philosophy of "one antigen, one technique, one paper".</p>https://oap.unige.ch/journals/abrep/article/view/644The AJ072 antibody against the human transferrin receptor labels HeLa cells by surface immunofluorescence2022-01-04T05:43:02+00:00Claudie Bianwanessa.delima@unige.ch<p class="Para"><span lang="EN-US">The AJ072 antibody against the human transferrin receptor labels the cell membrane of HeLa cells by surface immunofluorescence; AM236 does not.</span></p>2022-01-04T00:00:00+00:00Copyright (c) 2022 Claudie Bianhttps://oap.unige.ch/journals/abrep/article/view/677AF394, AF395, AF397, AK142, AK652, AN193 and AV441 antibodies label the GFP protein by western blot2022-02-17T09:13:21+00:00Cyril Guilhencyril.guilhen@unige.chMathieu Vigneaumathieu.vigneau@etu.unige.chBethania Tamratbethania.tamrat@etu.unige.chEmma Siebenmannemma.siebenmann@etu.unige.chTasnim Khadam-Al-JameTasnim.Khadam-Al-Jame@etu.unige.chHugo GilletHugo.Gillet@etu.unige.chEva GarridoEva.Garrido@etu.unige.chHania FarhatHania.Farhat@etu.unige.chJade BerlincourtJade.Berlincourt@etu.unige.chLuca BaltazarLuca.Baltazar@etu.unige.chTess ArbezTess.Arbez@etu.unige.chBenjamin AdomakoBenjamin.Adomako@etu.unige.chMaxime Zholdokovmaxime.zholdokov@etu.unige.chMarc Bobilliermarc.bobillier@etu.unige.chVincent Mendes Ferreiravincent.mendes@etu.unige.chAnja Maaganja.maag@etu.unige.chMaria Lungmaria.lung@etu.unige.chPatricia Lopezpatricia.lopez@etu.unige.chJulien Schärjulien.schaer@etu.unige.chYekaterina Prutyanovayekaterina.prutyanova@etu.unige.chMarin Ollagnonmarin.ollagnon@etu.unige.chGarance Michelgarance.michel@etu.unige.chAlexis Calomenialexis.calomeni@etu.unige.chAgnès Buryagnes.bury@etu.unige.chDiana Boevadiana.boeva@etu.unige.chStéphane Durualstephane.durual@unige.ch<p class="Para"><span lang="EN-US">The recombinant antibodies AF394, AF395, AF397, AK142, AK652, AN193 and AV441 detect the GFP protein by western blot.</span></p>2022-04-05T00:00:00+00:00Copyright (c) 2022 Cyril Guilhen, Vincent Mendes Ferreira, Anja Maag, Maria Lung, Patricia Lopez, Julien Schär, Yekaterina Prutyanova, Marin Ollagnon, Garance Michel, Alexis Calomeni, Agnès Bury, Diana Boeva, Marc Bobillier, Maxime Zholdokov, Mathieu Vigneau, Bethania Tamrat, Emma Siebenmann, Tasnim Khadam-Al-Jame, Hugo Gillet, Eva Garrido, Hania Farhat, Jade Berlincourt, Luca Baltazar, Tess Arbez, Benjamin Adomako, Stéphane Durualhttps://oap.unige.ch/journals/abrep/article/view/761The AK423 antibody recognizes human actin by western blot2022-04-12T06:41:19+00:00Anna Marchettiwanessa.delima@unige.ch<p>The AK423 antibody, raised against <em>Dictyostelium</em> actin, recognizes human actin by western blot; AE765, AK692, AO233 and AO234 do not.</p>2022-05-02T00:00:00+00:00Copyright (c) 2022 Anna Marchettihttps://oap.unige.ch/journals/abrep/article/view/671AG275, AG294 and AG274 antibodies recognize the hen egg-white lysozyme by ELISA2022-02-15T08:12:29+00:00Benjamin AdomakoBenjamin.Adomako@etu.unige.chVincent Mendes Ferreiravincent.mendes@etu.unige.chGarance Michelgarance.michel@etu.unige.chMarin Ollagnonmarin.ollagnon@etu.unige.chYekaterina Prutyanovayekaterina.prutyanova@etu.unige.chJulien Schaerjulien.schaer@etu.unige.chEmma Siebenmannemma.siebenmann@etu.unige.chBethania Tamratbethania.tamrat@etu.unige.chMathieu Vigneaumathieu.vigneau@etu.unige.chMaxime Zholdokovmaxime.zholdokov@etu.unige.chPhilippe HammelPhilippe.Hammel@unige.chStéphane Durualstéphane.durual@unige.chAnja Maaganja.maag@etu.unige.chMaria Lungmaria.lung@etu.unige.chPatricia Lopezpatricia.lopez@etu.unige.chTess ArbezTess.Arbez@etu.unige.chLuca BaltazarLuca.Baltazar@etu.unige.chJade BerlincourtJade.Berlincourt@etu.unige.chMarc BobillierMarc.Bobillier@etu.unige.chDiana BoevaDiana.Boeva@etu.unige.chAgnès BuryAgnes.Bury@etu.unige.chAlexis CalomeniAlexis.Calomeni@etu.unige.chHania Farhathania.farhat@etu.unige.chEva Garridoeva.garrido@etu.unige.chHugo Gillethugo.gillet@etu.unige.chTasnim Khadam-Al-Jametasnim.khadam-al-jame@etu.unige.chCyril Guilhencyril.guilhen@unige.ch<p class="Para"><span lang="EN-US">The recombinant antibodies AG275, AG294 and AG274 detect the hen egg-white lysozyme by ELISA.</span></p>2022-02-17T00:00:00+00:00Copyright (c) 2022 Benjamin Adomako, Tess Arbez, Luca Baltazar, Jade Berlincourt, Marc Bobillier, Diana Boeva, Agnès Bury, Alexis Calomeni, Hania Farhat, Eva Garrido, Hugo Gillet, Tasnim Khadam-Al-Jame, Patricia Lopez, Maria Lung, Anja Maag, Vincent Mendes Ferreira, Garance Michel, Marin Ollagnon, Yekaterina Prutyanova, Julien Schaer, Emma Siebenmann, Bethania Tamrat, Mathieu Vigneau, Maxime Zholdokov, Philippe Hammel, Stéphane Durual, Cyril Guilhenhttps://oap.unige.ch/journals/abrep/article/view/756The pemphigus monoclonal antibody clone P5G6 (AX353) retains cell-surface staining by immunofluorescence and desmoglein binding by ELISA in an engineered scFv-Fc format2022-04-08T18:04:13+00:00Christoph M. Hammershammers@web.deJens Wrammertjwramme@emory.edu<p class="Para"><span lang="EN-US">The recombinant human antibody clone P5G6 (AX353) in an engineered scFv-Fc format detects by immunofluorescence cell-surface proteins on cryosections of monkey esophagus. The same antibody detects by ELISA recombinant human desmoglein 3 and desmoglein 1 protein.</span></p>2022-04-19T00:00:00+00:00Copyright (c) 2022 Christoph M. Hammers, Jens Wrammerthttps://oap.unige.ch/journals/abrep/article/view/650The AJ072 antibody against the human transferrin receptor labels the surface and early endosomes of HEK cells by immunofluorescence2022-02-17T08:52:55+00:00Claudie Bianwanessa.delima@unige.ch<p class="Para"><span lang="EN-US">The AJ072 antibody, against the human transferrin receptor, labels the plasma membrane and early endosomes of HEK cells by surface and total immunofluorescence.</span></p>2022-02-23T00:00:00+00:00Copyright (c) 2022 Claudie Bianhttps://oap.unige.ch/journals/abrep/article/view/682The AI516 and AI523 antibodies recognize the Klebsiella pneumoniae KpGe strain by flow cytometry2022-02-24T08:53:19+00:00Xenia Crespo-Yanezwanessa.delima@unige.chImen Ayadiwanessa.delima@unige.ch<div class="page" title="Page 1"> <div class="layoutArea"> <div class="column"> <p>The recombinant antibodies AE516 and AI523 bind to the surface of the Klebsiella pneumoniae KpGe strain, as detected by flow cytometry. They do not bind to a K. pneumoniae strain defective in O-antigen synthesis.</p> </div> </div> </div>2022-03-03T00:00:00+00:00Copyright (c) 2022 Xenia Crespo-Yanez, Imen Ayadihttps://oap.unige.ch/journals/abrep/article/view/676AC650, AC653, AC656 and AD460 antibodies label the mouse CD8α protein by immunofluorescence2022-02-14T11:51:48+00:00Tasnim Khadam-Al-Jametasnim.khadam-al-jame@etu.unige.chHugo Gillethugo.gillet@etu.unige.chEva Garridoeva.garrido@etu.unige.chMaria Lung Perezmaria.lung@etu.unige.chJade Berlincourtjade.berlincourt@etu.unige.chMaxime Zholdokovmaxime.zholdokov@etu.unige.chMarin Ollagnonmarin.ollagnon@etu.unige.chAlexis Calomenialexis.calomeni@etu.unige.chPatricia Lopez Naveirapatricia.lopez@etu.unige.chBethania Tamratbethania.tamrat@etu.unige.chLuca Baltazarluca.baltazar@etu.unige.chGarance Michelgarance.michel@etu.unige.chAgnès Buryagnes.bury@etu.unige.chAnna Marchetti Anna.Marchetti@unige.chEmma Siebenmannemma.siebenmann@etu.unige.chTess Arbeztess.arbez@etu.unige.chVincent Mendes Ferreiravincent.mendes@etu.unige.chDiana Boevadiana.boeva@etu.unige.chJulien Schärjulien.schaer@etu.unige.chBenjamin Adomakobenjamin.adomako@etu.unige.chAnja Maaganja.maag@etu.unige.chMarc Bobilliermarc.bobillier@etu.unige.chMathieu Vigneaumathieu.vigneau@etu.unige.chYekaterina Prutyanovayekaterina.prutyanova@etu.unige.chHania Farhathania.farhat@etu.unige.chFrançois Prodonfrançois.prodon@unige.chStéphane Durualstéphane.durual@unige.chCyril Guilhencyril.guilhen@unige.ch<p class="Para"><span lang="EN-US">The recombinant antibodies AC650, AC653, AC656 and AD460 detect by immunofluorescence the mouse CD8</span><span lang="EN-US" style="font-family: Symbol;">α</span><span lang="EN-US"> protein.</span></p>2022-04-26T00:00:00+00:00Copyright (c) 2022 Cyril Guilhen, Tasnim Khadam-Al-Jame, Hugo Gillet, Eva Garrido, Hania Farhat, Alexis Calomeni, Agnès Bury, Diana Boeva, Marc Bobillier, Jade Berlincourt, Luca Baltazar, Tess Arbez, Benjamin Adomako, Maxime Zholdokov, Mathieu Vigneau, Bethania Tamrat, Emma Siebenmann, Julien Schär, Yekaterina Prutyanova, Marin Ollagnon, Garance Michel, Vincent Mendes Ferreira, Anja Maag, Maria Lung Perez, Patricia Lopez Naveira, Anna Marchetti , François Prodon, Stéphane Durualhttps://oap.unige.ch/journals/abrep/article/view/759The AE765, AK423, AK692, AO233 and AO234 antibodies recognize human actin by immunofluorescence in HEK cells2022-04-11T06:46:57+00:00Anna Marchettiwanessa.delima@unige.ch<p>The AE765, AK692, AO233 and AO234 antibodies recognize human actin in PAF-fixed HEK cells by immunofluorescence. Phalloidin, which recognizes polymerized actin, is more adequate to label actin filamentous structures. The AK423 antibody recognizes human actin in methanol-fixed HEK cells, but in these conditions the actin network is poorly preserved.</p>2022-05-06T00:00:00+00:00Copyright (c) 2022 Anna Marchettihttps://oap.unige.ch/journals/abrep/article/view/670AC650, AC653, AC656 and AD460 antibodies recognize the human CD8α protein by flow cytometry2022-02-11T15:35:47+00:00Maxime ZholdokovMaxime.Zholdokov@etu.unige.chMathieu VigneauMathieu.Vigneau@etu.unige.chBethania TamratBethania.Tamrat@etu.unige.chEmma SiebenmannEmma.Siebenmann@etu.unige.chJulien SchärJulien.Schaer@etu.unige.chYekaterina PrutyanovaYekaterina.Prutyanova@etu.unige.chMarin OllagnonMarin.Ollagnon@etu.unige.chGarance MichelGarance.Michel@etu.unige.chVincent Mendes FerreiraVincent.Mendes@etu.unige.chAnja MaagAnja.Maag@etu.unige.chMaria LungMaria.Lung@etu.unige.chPatricia LopezPatricia.Lopez@etu.unige.chTasnim Khadam-Al-JameTasnim.Khadam-Al-Jame@etu.unige.chHugo GilletHugo.Gillet@etu.unige.chEva GarridoEva.Garrido@etu.unige.chHania FarhatHania.Farhat@etu.unige.chAlexis CalomeniAlexis.Calomeni@etu.unige.chAgnès BuryAgnes.Bury@etu.unige.chDiana BoevaDiana.Boeva@etu.unige.chMarc BobillierMarc.Bobillier@etu.unige.chJade BerlincourtJade.Berlincourt@etu.unige.chLuca BaltazarLuca.Baltazar@etu.unige.chTess ArbezTess.Arbez@etu.unige.chBenjamin AdomakoBenjamin.Adomako@etu.unige.chAnna MarchettiAnna.Marchetti@unige.chStéphane Durualstephane.durual@unige.chJean-Pierre Aubry-Lachainayejean-pierre.aubry-lachainaye@unige.chCyril Guilhencyril.guilhen@unige.ch<div> <p class="Para"><span lang="EN-US">The recombinant antibodies AC650, AC653, AC656 and AD460 detect the human CD8</span><span lang="EN-US">α</span><span lang="EN-US"> protein by flow cytometry.</span></p> </div>2022-02-24T00:00:00+00:00Copyright (c) 2022 Maxime Zholdokov, Mathieu Vigneau, Bethania Tamrat, Emma Siebenmann, Julien Schär, Yekaterina Prutyanova, Marin Ollagnon, Garance Michel, Vincent Mendes Ferreira, Anja Maag, Maria Lung, Patricia Lopez, Tasnim Khadam-Al-Jame, Hugo Gillet, Eva Garrido, Hania Farhat, Alexis Calomeni, Agnès Bury, Diana Boeva, Marc Bobillier, Jade Berlincourt, Luca Baltazar, Tess Arbez, Benjamin Adomako, Anna Marchetti, Stéphane Durual, Jean-Pierre Aubry-Lachainaye, Cyril Guilhenhttps://oap.unige.ch/journals/abrep/article/view/725AI334, AQ806, AR222, AR249, AS274, AS702, AS708, RB590, RB591 and RB596 antibodies recognize the spike S protein from SARS-CoV-2 by western blot2022-03-29T10:30:10+00:00Anna Marchettiwanessa.delima@unige.chFrederic Zenhausernwanessa.delima@unige.ch<p>The recombinant antibodies AI334, AQ806, AR222, AR249, AS274, AS702, AS708, RB590, RB591 and RB596 detect by western blot the spike S protein from SARS-CoV-2.</p>2022-03-29T00:00:00+00:00Copyright (c) 2022 Anna Marchetti, Frederic Zenhausern