https://oap.unige.ch/journals/abrep/issue/feed Antibody Reports 2020-11-18T23:51:45+00:00 Prof. Pierre Cosson AntibodyReports@unige.ch Open Journal Systems <p><strong>Antibody Reports</strong> (ISSN 2624-8557) is an international, peer-reviewed open access online journal edited by the <a href="https://www.unige.ch/medecine/antibodies/" target="_blank" rel="noopener">Geneva Antibody Facility</a>, designed to focus on technical, experimental validation of recombinant antibodies. Our aim is to encourage scientists to publish their experimental results in as much detail as possible, with the philosophy of "one antigen, one technique, one paper".</p> https://oap.unige.ch/journals/abrep/article/view/220 AR222, AR249, AS274, AS702 and AS708 antibodies recognize the spike S protein from SARS-CoV-2 by ELISA 2020-11-09T23:44:45+00:00 Philippe Hammel wanessa.delima@unige.ch Anna Marchetti wanessa.delima@unige.ch Frederic Zenhausern wanessa.delima@unige.ch <p>The recombinant antibodies AI334, AQ806 and RB596 detect by immunofluorescence the spike S protein from SARS-CoV-2.</p> 2020-06-23T11:04:04+00:00 Copyright (c) 2020 The author(s) https://oap.unige.ch/journals/abrep/article/view/228 AR222, AR249, AS274, AS702 and AS708 antibodies recognize the spike S protein from SARS-CoV-2 by immunofluorescence 2020-11-09T23:43:52+00:00 Anna Marchetti wanessa.delima@unige.ch Frederic Zenhausern wanessa.delima@unige.ch <p>The recombinant antibodies AR222, AR249, AS274, AS702 and AS708 detect by immunofluorescence the spike S protein from SARS-CoV-2.</p> 2020-07-13T18:08:51+00:00 Copyright (c) 2020 The author(s) https://oap.unige.ch/journals/abrep/article/view/230 RB571, RB572, RB573, RB574, RB575, RB576, RB577 and RB578 antibodies recognize a fragment of the membrane M protein from SARS-CoV-2 by ELISA 2020-08-04T05:05:12+00:00 Philippe Hammel wanessa.delima@unige.ch Frederic Zenhausern wanessa.delima@unige.ch <p>The recombinant antibodies RB571, RB572, RB573, RB574, RB575, RB576, RB577 and RB578 detect by ELISA a synthetic peptide from the SARS-CoV-2 M protein.</p> 2020-07-09T04:21:14+00:00 Copyright (c) 2020 The author(s) https://oap.unige.ch/journals/abrep/article/view/231 RB572, RB574 and RB576 antibodies recognize the membrane M protein from SARS-CoV-2 by immunofluorescence 2020-11-09T23:43:26+00:00 Anna Marchetti wanessa.delima@unige.ch Frederic Zenhausern wanessa.delima@unige.ch <p>The recombinant antibodies RB572, RB574 and RB576 detect by immunofluorescence the spike S protein from SARS-CoV-2.</p> 2020-07-13T18:10:12+00:00 Copyright (c) 2020 The author(s) https://oap.unige.ch/journals/abrep/article/view/232 The RB596 antibody recognizes a linear epitope from the spike S protein from SARS-CoV-2 2020-11-09T23:44:19+00:00 Lluc Farrera-Soler wanessa.delima@unige.ch Jean-Pierre Daguer wanessa.delima@unige.ch Sofia Barluenga wanessa.delima@unige.ch Nicolas Winssinger wanessa.delima@unige.ch <p>The recombinant antibody RB596 recognizes a linear epitope (residues 973-984, ISSVLNDILSRL) from the SARS-CoV-2 spike S protein.</p> 2020-07-13T12:40:13+00:00 Copyright (c) 2020 The author(s) https://oap.unige.ch/journals/abrep/article/view/235 RB137 recognizes LL37 in neutrophil-extracellular trap-like (NET) structures in systemic lupus erythematosus and rheumatoid arthritis inflamed tissues by immunofluorescence in histological sections 2020-11-09T23:42:59+00:00 Roberto Lande wanessa.delima@unige.ch Stefano Alivernini wanessa.delima@unige.ch Barbara Tolusso wanessa.delima@unige.ch Francesca Spadaro wanessa.delima@unige.ch Mario Falchi wanessa.delima@unige.ch Raffaella Palazzo wanessa.delima@unige.ch Immacolata Pietraforte wanessa.delima@unige.ch Carlo Chizzolini wanessa.delima@unige.ch Kostantino Giannakakis wanessa.delima@unige.ch Francesca Romana Spinelli wanessa.delima@unige.ch Cristiano Alessandri wanessa.delima@unige.ch Elisa Gremese wanessa.delima@unige.ch Fabrizio Conti wanessa.delima@unige.ch Loredana Frasca wanessa.delima@unige.ch <p>Besides being a natural antibiotic, the human cathelicidin LL37, produced by epithelial cells and neutrophils, is an important immune-modulator. LL37 alone, or in complex with DNA, can activate inflammatory pathways in psoriasis, Systemic Lupus Erythematosus (SLE) and Rheumatoid Arthritis (RA). In this work, we describe the capacity of the recombinant monoclonal antibody RB137, previously shown to specifically recognize LL37 in its native form by ELISA, to detect LL37 by immunofluorescence in human tissues derived from SLE and RA patients. In these tissues, LL37 decorates DNA filaments resembling neutrophil-extracellular-trap (NET) structures. This antibody represents a valuable tool to detect the presence, the native state and the exact localization of LL37 in human tissues in health and disease.</p> 2020-07-27T04:19:58+00:00 Copyright (c) 2020 The author(s) https://oap.unige.ch/journals/abrep/article/view/236 Monoclonal antibodies RB139 and RB142 recognize citrullinated LL37 by immunofluorescence in histological sections in Systemic lupus erythematosus (SLE) and Rheumatoid arthritis (RA) 2020-11-09T23:42:31+00:00 Roberto Lande wanessa.delima@unige.ch Stefano Alivernini wanessa.delima@unige.ch Barbara Tolusso wanessa.delima@unige.ch Francesca Spadaro wanessa.delima@unige.ch Mario Falchi wanessa.delima@unige.ch Raffaella Palazzo wanessa.delima@unige.ch Immacolata Pietraforte wanessa.delima@unige.ch Carlo Chizzolini wanessa.delima@unige.ch Kostantino Giannakakis wanessa.delima@unige.ch Francesca Romana Spinelli wanessa.delima@unige.ch Cristiano Alessandri wanessa.delima@unige.ch Fabrizio Conti wanessa.delima@unige.ch Elisa Gremese wanessa.delima@unige.ch Loredana Frasca wanessa.delima@unige.ch <p>LL37 is a natural antibiotic, but is also a molecule with pleiotropic functions as well as an immune-modulator. LL37 is produced by epithelial cells and is present in neutrophils’ granules. LL37 alone, or in complex with DNA, can activate inflammatory pathways in psoriasis, systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). In this work, we describe the capacity of two recombinant monoclonal antibodies, RB139 and RB142, previously shown to specifically recognize LL37 in its citrullinated form (cit-LL37) by ELISA, to detect LL37 by immunofluorescence in human inflamed tissues derived from SLE and RA patients. Such antibodies represent previously unavailable tools to detect the presence, the citrullinated state and the exact localization of cit-LL37 in human tissues in health and disease.</p> 2020-07-27T04:21:01+00:00 Copyright (c) 2020 The author(s) https://oap.unige.ch/journals/abrep/article/view/238 RB620 and RB621 antibodies recognize a peptide of the SARS-CoV-2 E protein by ELISA 2020-11-09T23:42:03+00:00 Philippe Hammel wanessa.delima@unige.ch Frederic Zenhausern wanessa.delima@unige.ch <p>The recombinant antibodies RB620 and RB621 detect by ELISA a synthetic peptide from the SARS-CoV-2 envelope E protein.</p> 2020-08-24T10:33:32+00:00 Copyright (c) 2020 The author(s) https://oap.unige.ch/journals/abrep/article/view/239 The RB621 antibody recognizes the envelope E protein from SARS-CoV-2 by immunofluorescence 2020-11-09T23:41:38+00:00 Anna Marchetti wanessa.delima@unige.ch Frederic Zenhausern wanessa.delima@unige.ch <p>The recombinant antibody RB621 detects by immunofluorescence the envelope E protein from SARS-CoV-2.</p> 2020-08-26T05:10:14+00:00 Copyright (c) 2020 The author(s) https://oap.unige.ch/journals/abrep/article/view/240 AG424 and AG427 antibodies recognize the human TrkB protein by western blot 2020-11-09T23:41:12+00:00 Audrey Roussel-Gervais wanessa.delima@unige.ch Stéphanie Sgroi wanessa.delima@unige.ch Vincent Jaquet wanessa.delima@unige.ch <p>The recombinant antibodies AG424 and AG427 detect the human TrkB protein in the human neural progenitor cell line ReNcell<sup>® </sup>VM and in human brain by western blot.</p> <div id="gtx-trans" style="position: absolute; left: 114px; top: 36px;"> <div class="gtx-trans-icon">&nbsp;</div> </div> 2020-09-04T10:19:04+00:00 Copyright (c) 2020 The author(s) https://oap.unige.ch/journals/abrep/article/view/260 The AA345 antibody specifically recognizes α-tubulin by western blot in human cell lines 2020-11-18T23:51:45+00:00 Lucie Oberhauser wanessa.delima@unige.ch Miriam Stoeber wanessa.delima@unige.ch <p>The recombinant antibody AA345 specifically detects human α-tubulin in HEK293, Hela, and HepG2 human cell lines by western blot.</p> 2020-11-17T07:05:21+00:00 Copyright (c) 2020 The author(s)