https://oap.unige.ch/journals/abrep <p><strong>Antibody Reports</strong> (eISSN 2624-8557) is an international, peer-reviewed open access online journal edited by the <a href="https://www.unige.ch/medecine/antibodies/" target="_blank" rel="noopener">Geneva Antibody Facility</a>, designed to focus on technical, experimental validation of recombinant antibodies. Our aim is to encourage scientists to publish their experimental results in as much detail as possible, with the philosophy of "one antigen, one technique, one paper".</p> Bibliothèque de l'Université de Genève en-US Antibody Reports 2624-8557 https://oap.unige.ch/journals/abrep/article/view/644 <p class="Para"><span lang="EN-US">The AJ072 antibody against the human transferrin receptor labels the cell membrane of HeLa cells by surface immunofluorescence; AM236 does not.</span></p> Claudie Bian Copyright (c) 2022 Claudie Bian https://creativecommons.org/licenses/by/4.0 2022-01-04 2022-01-04 5 1 e644 e644 10.24450/journals/abrep.2022.e644 https://oap.unige.ch/journals/abrep/article/view/677 <p class="Para"><span lang="EN-US">The recombinant antibodies AF394, AF395, AF397, AK142, AK652, AN193 and AV441 detect the GFP protein by western blot.</span></p> Cyril Guilhen Mathieu Vigneau Bethania Tamrat Emma Siebenmann Tasnim Khadam-Al-Jame Hugo Gillet Eva Garrido Hania Farhat Jade Berlincourt Luca Baltazar Tess Arbez Benjamin Adomako Maxime Zholdokov Marc Bobillier Vincent Mendes Ferreira Anja Maag Maria Lung Patricia Lopez Julien Schär Yekaterina Prutyanova Marin Ollagnon Garance Michel Alexis Calomeni Agnès Bury Diana Boeva Stéphane Durual Copyright (c) 2022 Cyril Guilhen, Vincent Mendes Ferreira, Anja Maag, Maria Lung, Patricia Lopez, Julien Schär, Yekaterina Prutyanova, Marin Ollagnon, Garance Michel, Alexis Calomeni, Agnès Bury, Diana Boeva, Marc Bobillier, Maxime Zholdokov, Mathieu Vigneau, Bethania Tamrat, Emma Siebenmann, Tasnim Khadam-Al-Jame, Hugo Gillet, Eva Garrido, Hania Farhat, Jade Berlincourt, Luca Baltazar, Tess Arbez, Benjamin Adomako, Stéphane Durual https://creativecommons.org/licenses/by/4.0 2022-04-05 2022-04-05 5 1 e677 e677 10.24450/journals/abrep.2022.e677 https://oap.unige.ch/journals/abrep/article/view/761 <p>The AK423 antibody, raised against <em>Dictyostelium</em> actin, recognizes human actin by western blot; AE765, AK692, AO233 and AO234 do not.</p> Anna Marchetti Copyright (c) 2022 Anna Marchetti https://creativecommons.org/licenses/by/4.0 2022-05-02 2022-05-02 5 1 e761 e761 10.24450/journals/abrep.2022.e761 https://oap.unige.ch/journals/abrep/article/view/671 <p class="Para"><span lang="EN-US">The recombinant antibodies AG275, AG294 and AG274 detect the hen egg-white lysozyme by ELISA.</span></p> Benjamin Adomako Vincent Mendes Ferreira Garance Michel Marin Ollagnon Yekaterina Prutyanova Julien Schaer Emma Siebenmann Bethania Tamrat Mathieu Vigneau Maxime Zholdokov Philippe Hammel Stéphane Durual Anja Maag Maria Lung Patricia Lopez Tess Arbez Luca Baltazar Jade Berlincourt Marc Bobillier Diana Boeva Agnès Bury Alexis Calomeni Hania Farhat Eva Garrido Hugo Gillet Tasnim Khadam-Al-Jame Cyril Guilhen Copyright (c) 2022 Benjamin Adomako, Tess Arbez, Luca Baltazar, Jade Berlincourt, Marc Bobillier, Diana Boeva, Agnès Bury, Alexis Calomeni, Hania Farhat, Eva Garrido, Hugo Gillet, Tasnim Khadam-Al-Jame, Patricia Lopez, Maria Lung, Anja Maag, Vincent Mendes Ferreira, Garance Michel, Marin Ollagnon, Yekaterina Prutyanova, Julien Schaer, Emma Siebenmann, Bethania Tamrat, Mathieu Vigneau, Maxime Zholdokov, Philippe Hammel, Stéphane Durual, Cyril Guilhen https://creativecommons.org/licenses/by/4.0 2022-02-17 2022-02-17 5 1 e671 e671 10.24450/journals/abrep.2022.e671 https://oap.unige.ch/journals/abrep/article/view/756 <p class="Para"><span lang="EN-US">The recombinant human antibody clone P5G6 (AX353) in an engineered scFv-Fc format detects by immunofluorescence cell-surface proteins on cryosections of monkey esophagus. The same antibody detects by ELISA recombinant human desmoglein 3 and desmoglein 1 protein.</span></p> Christoph M. Hammers Jens Wrammert Copyright (c) 2022 Christoph M. Hammers, Jens Wrammert https://creativecommons.org/licenses/by/4.0 2022-04-19 2022-04-19 5 1 e756 e756 10.24450/journals/abrep.2022.e756 https://oap.unige.ch/journals/abrep/article/view/650 <p class="Para"><span lang="EN-US">The AJ072 antibody, against the human transferrin receptor, labels the plasma membrane and early endosomes of HEK cells by surface and total immunofluorescence.</span></p> Claudie Bian Copyright (c) 2022 Claudie Bian https://creativecommons.org/licenses/by/4.0 2022-02-23 2022-02-23 5 1 e650 e650 10.24450/journals/abrep.2022.e650 https://oap.unige.ch/journals/abrep/article/view/682 <div class="page" title="Page 1"> <div class="layoutArea"> <div class="column"> <p>The recombinant antibodies AE516 and AI523 bind to the surface of the Klebsiella pneumoniae KpGe strain, as detected by flow cytometry. They do not bind to a K. pneumoniae strain defective in O-antigen synthesis.</p> </div> </div> </div> Xenia Crespo-Yanez Imen Ayadi Copyright (c) 2022 Xenia Crespo-Yanez, Imen Ayadi https://creativecommons.org/licenses/by/4.0 2022-03-03 2022-03-03 5 1 e682 e682 10.24450/journals/abrep.2022.e682 https://oap.unige.ch/journals/abrep/article/view/676 <p class="Para"><span lang="EN-US">The recombinant antibodies AC650, AC653, AC656 and AD460 detect by immunofluorescence the mouse CD8</span><span lang="EN-US" style="font-family: Symbol;">α</span><span lang="EN-US"> protein.</span></p> Tasnim Khadam-Al-Jame Hugo Gillet Eva Garrido Maria Lung Perez Jade Berlincourt Maxime Zholdokov Marin Ollagnon Alexis Calomeni Patricia Lopez Naveira Bethania Tamrat Luca Baltazar Garance Michel Agnès Bury Anna Marchetti Emma Siebenmann Tess Arbez Vincent Mendes Ferreira Diana Boeva Julien Schär Benjamin Adomako Anja Maag Marc Bobillier Mathieu Vigneau Yekaterina Prutyanova Hania Farhat François Prodon Stéphane Durual Cyril Guilhen Copyright (c) 2022 Cyril Guilhen, Tasnim Khadam-Al-Jame, Hugo Gillet, Eva Garrido, Hania Farhat, Alexis Calomeni, Agnès Bury, Diana Boeva, Marc Bobillier, Jade Berlincourt, Luca Baltazar, Tess Arbez, Benjamin Adomako, Maxime Zholdokov, Mathieu Vigneau, Bethania Tamrat, Emma Siebenmann, Julien Schär, Yekaterina Prutyanova, Marin Ollagnon, Garance Michel, Vincent Mendes Ferreira, Anja Maag, Maria Lung Perez, Patricia Lopez Naveira, Anna Marchetti , François Prodon, Stéphane Durual https://creativecommons.org/licenses/by/4.0 2022-04-26 2022-04-26 5 1 e676 e676 10.24450/journals/abrep.2022.e676 https://oap.unige.ch/journals/abrep/article/view/759 <p>The AE765, AK692, AO233 and AO234 antibodies recognize human actin in PAF-fixed HEK cells by immunofluorescence. Phalloidin, which recognizes polymerized actin, is more adequate to label actin filamentous structures. The AK423 antibody recognizes human actin in methanol-fixed HEK cells, but in these conditions the actin network is poorly preserved.</p> Anna Marchetti Copyright (c) 2022 Anna Marchetti https://creativecommons.org/licenses/by/4.0 2022-05-06 2022-05-06 5 1 e759 e759 10.24450/journals/abrep.2022.e759 https://oap.unige.ch/journals/abrep/article/view/670 <div> <p class="Para"><span lang="EN-US">The recombinant antibodies AC650, AC653, AC656 and AD460 detect the human CD8</span><span lang="EN-US">α</span><span lang="EN-US"> protein by flow cytometry.</span></p> </div> Maxime Zholdokov Mathieu Vigneau Bethania Tamrat Emma Siebenmann Julien Schär Yekaterina Prutyanova Marin Ollagnon Garance Michel Vincent Mendes Ferreira Anja Maag Maria Lung Patricia Lopez Tasnim Khadam-Al-Jame Hugo Gillet Eva Garrido Hania Farhat Alexis Calomeni Agnès Bury Diana Boeva Marc Bobillier Jade Berlincourt Luca Baltazar Tess Arbez Benjamin Adomako Anna Marchetti Stéphane Durual Jean-Pierre Aubry-Lachainaye Cyril Guilhen Copyright (c) 2022 Maxime Zholdokov, Mathieu Vigneau, Bethania Tamrat, Emma Siebenmann, Julien Schär, Yekaterina Prutyanova, Marin Ollagnon, Garance Michel, Vincent Mendes Ferreira, Anja Maag, Maria Lung, Patricia Lopez, Tasnim Khadam-Al-Jame, Hugo Gillet, Eva Garrido, Hania Farhat, Alexis Calomeni, Agnès Bury, Diana Boeva, Marc Bobillier, Jade Berlincourt, Luca Baltazar, Tess Arbez, Benjamin Adomako, Anna Marchetti, Stéphane Durual, Jean-Pierre Aubry-Lachainaye, Cyril Guilhen https://creativecommons.org/licenses/by/4.0 2022-02-24 2022-02-24 5 1 e670 e670 10.24450/journals/abrep.2022.e670 https://oap.unige.ch/journals/abrep/article/view/725 <p>The recombinant antibodies AI334, AQ806, AR222, AR249, AS274, AS702, AS708, RB590, RB591 and RB596 detect by western blot the spike S protein from SARS-CoV-2.</p> Anna Marchetti Frederic Zenhausern Copyright (c) 2022 Anna Marchetti, Frederic Zenhausern https://creativecommons.org/licenses/by/4.0 2022-03-29 2022-03-29 5 1 e725 e725 10.24450/journals/abrep.2022.e725