AD946 and AF371 antibodies recognize a His-tagged recombinant protein by immunofluorescence

Authors

  • Wanessa C Lima
  • Pierre Cosson

DOI:

https://doi.org/10.24450/journals/abrep.2020.e134

Abstract

AD946 and AF371 antibodies against the His tag recognize a His-tagged human TAC protein by immunofluorescence in paraformaldehyde-fixed HeLa cells.

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References

Arsimoles D, D’Esposito A, Gaspoz V, et al. The AJ519 antibody labels the human TAC/IL2RA protein by immunofluorescence. Antibody Reports, 2020, 3:e118. doi:10.22450/journals/abrep.2020.e118

Hochuli E, Bannwarth W, Döbeli H, Gentz R, Stüber D. Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent. Bio/Technology 1998; 6:1321–1325. doi:10.1038/nbt1188-1321

Lima WC, Gasteiger E, Marcatili P, Duek P, Bairoch A, Cosson P. The ABCD database: a repository for chemically defined antibodies. Nucleic Acids Res. 2020; 48(D1):D261-D264. PMID:31410491

Lindner P, Bauer K, Krebber A, et al. Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions. Biotechniques 1997; 22(1):140-9. PMID:8994661

Shimizu M, Kajitani K. Anti-polyhistidine-tag antibody and method for avoiding nonspecific reaction by using the same. Japan; JP2011016773, 2011.

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Published

2020-02-13

How to Cite

1.
Lima WC, Cosson P. AD946 and AF371 antibodies recognize a His-tagged recombinant protein by immunofluorescence. Antib. Rep. [Internet]. 2020 Feb. 13 [cited 2022 Dec. 2];3(2):e134. Available from: https://oap.unige.ch/journals/abrep/article/view/134

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