AF394, AF395 and AF396 antibodies recognize a GFP-tagged recombinant protein by immunofluorescence

Authors

  • Wanessa C Lima
  • Pierre Cosson

DOI:

https://doi.org/10.24450/journals/abrep.2020.e131

Abstract

AF394, AF395 and AF396 antibodies against the GFP protein recognize a GFP-tagged human TAC protein by immunofluorescence in paraformaldehyde-fixed HeLa cells.

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References

Arsimoles D, D’Esposito A, Gaspoz V, et al. The AJ519 antibody labels the human TAC/IL2RA protein by immunofluorescence. Antibody Reports, 2020, 3:e118. doi:10.22450/journals/abrep.2020.e118

Kirchhofer A, Helma J, Schmidthals K, et al. Modulation of protein properties in living cells using nanobodies. Nat Struct Mol Biol. 2010; 17(1):133-8. PMID:20010839

Lima WC, Gasteiger E, Marcatili P, Duek P, Bairoch A, Cosson P. The ABCD database: a repository for chemically defined antibodies. Nucleic Acids Res. 2020; 48(D1):D261-D264. PMID:31410491

Rothbauer U, Zolghadr K, Tillib S, et al. Targeting and tracing antigens in live cells with fluorescent nanobodies. Nat Methods. 2006; 3(11):887-9. PMID:17060912

Tsien RY. The green fluorescent protein. Annu Rev Biochem. 1998; 67:509-44. PMID:9759496

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Published

2020-02-13

How to Cite

1.
Lima WC, Cosson P. AF394, AF395 and AF396 antibodies recognize a GFP-tagged recombinant protein by immunofluorescence. Antib. Rep. [Internet]. 2020 Feb. 13 [cited 2022 Dec. 2];3(2):e131. Available from: https://oap.unige.ch/journals/abrep/article/view/131

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