The RB008 antibody does not recognize D. discoideum NoxA by western blot

Florence Leuba; Thierry Soldati

doi:10.24450/journals/abrep.2020.e190

The RB008 antibody does not recognize D. discoideum NoxA by western blot

Authors

Florence Leuba
Thierry Soldati

DOI:

https://doi.org/10.24450/journals/abrep.2020.e190

Abstract

The recombinant antibody RB008 does not detect by western blot the Dictyostelium discoideum protein NoxA expressed in D. discoideum and in two human cell-lines.

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References

Dunn JD, Bosmani C, Barisch C, et al. Eat, prey, live: Dictyostelium discoideum as a model for cell-autonomous defenses. Front Immunol. 2018; 8:1906. PMID:29354124

Pedruzzi E, Fay M, Elbim C, Gaudry M, Gougerot-Pocidalo MA. Differentiation of PLB-985 myeloid cells into mature neutrophils, shown by degranulation of terminally differentiated compartments in response to N-formyl peptide and priming of superoxide anion production by granulocyte-macrophage colony-stimulating factor. Br J Haematol. 2002; 117:719-26. PMID:12028049

Watts DJ, Ashworth JM. Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochem J. 1970; 119:171-4. PMID:5530748

Zhang X, Zhuchenko O, Kuspa A, Soldati T. Social amoebae trap and kill bacteria by casting DNA nets. Nat Commun. 2016; 7:10938. PMID:26927887

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Published

2020-05-20 — Updated on 2023-03-03

Versions

2023-03-03 (2)
2020-05-20 (1)

How to Cite

Leuba F, Soldati T. The RB008 antibody does not recognize D. discoideum NoxA by western blot. Antib. Rep. [Internet]. 2023 Mar. 3 [cited 2023 Jun. 1];3(3):e190. Available from: https://oap.unige.ch/journals/abrep/article/view/190