AI842, AI843, AI844 and AI177 antibodies do not recognize a FLAG-tagged protein expressed in D. discoideum by western blot

Florence Leuba; Thierry Soldati

doi:10.24450/journals/abrep.2020.e111

AI842, AI843, AI844 and AI177 antibodies do not recognize a FLAG-tagged protein expressed in D. discoideum by western blot

Authors

Florence Leuba
Thierry Soldati University of Geneva https://orcid.org/0000-0002-2056-7931

DOI:

https://doi.org/10.24450/journals/abrep.2020.e111

Abstract

The recombinant antibodies AI842, AI843, AI844 and AI177 do not detect by western blot an N-terminal FLAG-tagged RavZ protein expressed in Dictyostelium discoideum.

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References

Choy A, Dancourt J, Mugo B, et al. The Legionella effector RavZ inhibits host autophagy through irreversible Atg8 deconjugation. Science, 2012; 338:1072–1076. PMID: 23112293

Entzminger KC, Hyun JM, Pantazes RJ, et al. De novo design of antibody complementarity determining regions binding a FLAG tetra-peptide. Sci Rep. 2017; 7(1):10295. PMID: 28860479

Lima WC, Gasteiger E, Marcatili P, Duek P, Bairoch A, Cosson P. The ABCD database: a repository for chemically defined antibodies. Nucleic Acids Res. 2019; pii:gkz714. PMID: 31410491

Sasaki F, Okuno T, Saeki K, et al. A high-affinity monoclonal antibody against the FLAG tag useful for G-protein-coupled receptor study. Anal Biochem. 2012; 425(2):157-65. PMID: 22465329

Veltman DM, Akar G, Bosgraaf L, Van Haastert PJM. A new set of small, extrachromosomal expression vectors for Dictyostelium discoideum. Plasmid, 2009; 61:110–118. PMID: 19063918

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Published

2020-01-15 — Updated on 2023-02-22

Versions

2023-02-22 (2)
2020-01-15 (1)

How to Cite

Leuba F, Soldati T. AI842, AI843, AI844 and AI177 antibodies do not recognize a FLAG-tagged protein expressed in D. discoideum by western blot. Antib. Rep. [Internet]. 2023 Feb. 22 [cited 2023 Jun. 1];3(1):e111. Available from: https://oap.unige.ch/journals/abrep/article/view/111