AI842, AI843, AI844 and AI177 antibodies do not recognize a FLAG-tagged protein by immunofluorescence in D. discoideum cells

Authors

DOI:

https://doi.org/10.24450/journals/abrep.2020.e126

Abstract

The recombinant antibodies AI842, AI843, AI844 and AI177 failed to detect by immunofluorescence a C-terminally FLAG-tagged LmpA fusion protein expressed in Dictyostelium discoideum.

References

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Lima WC, Gasteiger E, Marcatili P, Duek P, Bairoch A, Cosson P. The ABCD database: a repository for chemically defined antibodies. Nucleic Acids Res. 2019; pii:gkz714. PMID: 31410491

Sasaki F, Okuno T, Saeki K, et al. A high-affinity monoclonal antibody against the FLAG tag useful for G-protein-coupled receptor study. Anal Biochem. 2012; 425(2):157-65. PMID: 22465329

Sattler N, Bosmani C, Barisch, C, et al. Functions of the Dictyostelium LIMP-2 and CD36 homologues in bacteria uptake, phagolysosome biogenesis and host cell defence. J Cell Sci. 2018; 131(17):jcs218040. PMID: 30054386.

Veltman DM, Akar G, Bosgraaf L, Van Haastert PJ. A new set of small, extrachromosomal expression vectors for Dictyostelium discoideum. Plasmid. 2009; 61(2):110-8. PMID: 19063918.

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Published

2020-03-20

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Article

How to Cite

1.
Mottet M, Soldati T. AI842, AI843, AI844 and AI177 antibodies do not recognize a FLAG-tagged protein by immunofluorescence in D. discoideum cells. Antib. Rep. [Internet]. 2020 Mar. 20 [cited 2024 Apr. 21];3(2):e126. Available from: https://oap.unige.ch/journals/abrep/article/view/126