AI842, AI843, AI844 and AI177 antibodies do not recognize a FLAG-tagged protein by immunofluorescence in D. discoideum cells
DOI:
https://doi.org/10.24450/journals/abrep.2020.e126Abstract
The recombinant antibodies AI842, AI843, AI844 and AI177 failed to detect by immunofluorescence a C-terminally FLAG-tagged LmpA fusion protein expressed in Dictyostelium discoideum.
References
Entzminger KC, Hyun JM, Pantazes RJ, et al. De novo design of antibody complementarity determining regions binding a FLAG tetra-peptide. Sci Rep. 2017; 7(1):10295. PMID: 28860479
Hagedorn M, Neuhaus EM, Soldati T. Optimized fixation and immunofluorescence staining methods for Dictyostelium cells. Methods Mol Biol. 2006; 346:327-38. PMID: 16957300.
Lima WC, Gasteiger E, Marcatili P, Duek P, Bairoch A, Cosson P. The ABCD database: a repository for chemically defined antibodies. Nucleic Acids Res. 2019; pii:gkz714. PMID: 31410491
Sasaki F, Okuno T, Saeki K, et al. A high-affinity monoclonal antibody against the FLAG tag useful for G-protein-coupled receptor study. Anal Biochem. 2012; 425(2):157-65. PMID: 22465329
Sattler N, Bosmani C, Barisch, C, et al. Functions of the Dictyostelium LIMP-2 and CD36 homologues in bacteria uptake, phagolysosome biogenesis and host cell defence. J Cell Sci. 2018; 131(17):jcs218040. PMID: 30054386.
Veltman DM, Akar G, Bosgraaf L, Van Haastert PJ. A new set of small, extrachromosomal expression vectors for Dictyostelium discoideum. Plasmid. 2009; 61(2):110-8. PMID: 19063918.
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