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The AF122 antibody recognizes the AChR subunit in murine muscle endplates by immunofluorescence

Authors

  • Florian Ingelfinger
  • Dilay Cansever
  • Bettina Schreiner

DOI:

https://doi.org/10.24450/journals/abrep.2020.e112

Abstract

The recombinant antibody AF122 binds to the acetylcholine receptor (AChR) a subunit expressed in murine muscle endplates and can be detected by immunofluorescence in paraformaldehyde (PFA)-fixed tibialis anterior cryosections.

References

Graus YF, De Baets MH, Van Breda Vriesman PJ, Burton DR. Anti-acetylcholine receptor Fab fragments isolated from thymus-derived phage display libraries from myasthenia gravis patients reflect predominant specificities in serum and block the action of pathogenic serum antibodies. J Immunol. 1997; 158(4):1919-29. PMID: 9029134

Tzartos SJ, Changeux JP. High affinity binding of alpha-bungarotoxin to the purified alpha-subunit and to its 27,000-dalton proteolytic peptide from Torpedo marmorata acetylcholine receptor. Requirement for sodium dodecyl sulfate. EMBO J. 1983; 2(3):381–387. PMID: 11894953

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2020-01-14

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How to Cite

1.
Ingelfinger F, Cansever D, Schreiner B. The AF122 antibody recognizes the AChR subunit in murine muscle endplates by immunofluorescence. Antib. Rep. [Internet]. 2020 Jan. 14 [cited 2023 Dec. 10];3(1):e112. Available from: https://oap.unige.ch/journals/abrep/article/view/112

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